5505
ZeinabKhaled Saad Hamza
Controlof Mycotoxin Using Nanopolymers Prepared Fromnatural Sources
Yeastcellwall(YCW),aflatoxin b1(AFB1), glucanmannan lipid particle
(GMLP), nanoparticles(NPs), microencapsulation, mycotoxin binders
(MB),humicacid (HA),invitrogastrointestinalmodels,stability,
cytotoxicityandinvivostudies
AflatoxinB1 (AFB1)isoneofthemostpotentnaturallyoccurring hepatic carcinogensto bothhumanandanimals.The presentstudyaimed to assess the effect of encapsulated mycotoxin binders (detoxifying agents)insideyeastcellwalls(YCW)todetoxify AFB1invitrousingthe gastrointestinalmodels. AsyeastcellwallshavebeenpreviouslyreportedtobindAFB1. Theeightdifferentyeastcellwall-basedmaterialswere testedinvitrofor AFB1binding. TheresultsshowedthatGlucanMannanLipidParticles(GMLPs) fromSaccharomyces cerevisiae cell walls recorded thehighest AFB1 adsorption0.2µgAFB1/mgGMLPinsimulatedgastricfluid(SGF)after 10min,and0.17µg AFB1/mgGMLPin simulatedintestinalfluid(SIF) after 1h.GMLPsare hollow3-4micronporousmicrospheresthatprovide anefficientsystemfor thesynthesisandencapsulationofAFB1-absorbing nanoparticles(NPs). Humicacidnanoparticles(HA-NPs)were synthesizedwithinthe GMLPcavity bycomplexationwithferricchloride.Thehybrid (GMLP/HA FeNPs)synergisticallyenhancedAFB1bindingcomparedto unencapsulatedHANPsandotherAFB1absorbingmaterials.Asaresult, thehybrid GMLPHA-NPformulation synergisticallyenhancedAFB1 binding 1.6– 6.5fold compared to individualGMLPandHAcomponents inSGFandhigherthan9foldinSIF.Cellularcytotoxicity studies demonstratedthatGMLPHA-NP-AFB1 complexeswerestableinboth SGF andSIF andhave the abilitytodetoxifyAFB1119-57fold. Thecurrentdataofinvivostudiescleared thatAFB1at80mg/kg b.wt is multiorgans toxicants due to its adverse effects on liver and kidney.Theformulation(GMLP/HA FeNPs)atlevel0.5mg/kgb.wtwas effective inpartially reversingmycotoxicosisandalleviatinglossesin relationtothenegative control.
2018
Ph.d
Ain Shams
Science