5494
Hanan Samir Salah El- Din Ali
Molecular And Physiological Studies On Transformation Of Vicia Faba L. To Enhance Tolerance To Salt Stress
Agrobacterium mediated-transformation, AtNHX1,Na+/H+ antiporter, Vicia faba L., salinity
Arabidopsis thaliana AtNHX1 was previously identified as an important salt tolerance gene that transports Na+ into vacuoles. In the present study, AtNHX1 was transferred into the cells of the shoot apices of three Egyptian faba bean (Vicia faba L.) cultivars (Egypt 1, Giza 40 and Giza 843) via Agrobacterium-mediated transformation. Transgenic V. faba plantlets containing AtNHX1 and the selective marker encoding bialophos resistance (bar) were confirmed using (PCR). The expression of AtNHX1 in transgenic plants was confirmed by real-time PCR and western blot which indicated that the gene was highly expressed in transgenic plants. Physiological changes in transgenic and control plants of the three faba bean cultivars were examined in response to 0, 50, 100 and 200 mM NaCl. The obtained transgenic plants which were exposed to NaCl showed high levels of photosynthetic pigments, proline and Na+ but less malondialdehyde and K+ as compared to controls. In addition, salinity stress significantly increased the total antioxidant capacity and total phenol content in transgenic as compared to control plants. Our results demonstrate the value of introducing AtNHX1 into Vicia faba for improving salt stress.
2018
Ph.d
Ain Shams
Science