5091
HEND MOHAMMED EL- SAYED MANDOUR
GENETIC ANALYSIS AND IN VITRO SELECTION FOR DROUGHT TOLERANCE IN WHEAT (Triticum aestivum L.)
IN VITROSELECTION, DROUGHT, uhat, PEG, embryo attuk
Selection for drought tolerance of fifteen wheat genotypes (five parents and their ten F1 hybrids) was performed under field and laboratory conditions and data were analyzed by diallel analysis. Under laboratory conditions, three different callus induction media were used to determine the optimum hormone balance for callus induction from mature embryos wheat genotypes and also to study the genetic response of studied wheat genotypes to callus induction. MS media supplemented with different concentrations of poly ethylene glycol (PEG) were used to evaluate the obtained calli for drought tolerance. Then the drought stressed calli were then transferred to plant regeneration medium for studying their ability to regenerate. All characters were shown to be controlled by over dominance genetic except main spike length (cm) under normal conditions and plant height (cm) under drought stress conditions where, they were controlled with partial dominance genetic effect. Narrow-sense heritability was high for main spike length (cm) under both conditions, days to heading ,number of spikes / plant and 1000-grain weight (g) under normal conditions, plant height (cm), number of spikelets / spike and main spike yield (g) under drought stress conditions . M2 medium (2mg/l 2,4D+300 mg/l casein hydrolysate) gave thehighest callus induction frequency (85.5%) followed by M1 (2mg/l 2,4D) medium (85%) and M3 (2mg/l 2,4D +4 mg/l AgNo3) medium (81.6%). The differences between the three callus induction media were significant for all characters except callus induction frequency (CIF %) and M2 medium was the bestmedia for callus induction. The variety Gemmeiza10 gave the highest callus induction frequency while the variety (Misr1) possessed the lowest performance for the same character over the three studied media.
2016
M.Sc
Zagazig
Agriculture