4940
Hagar Hassan El Sayed Mourad
An Immunological Study on a Possible Protective Role of Calendula officinalis and Hypericum perforatum Against Trichloroethylene Induced liver Inflammation in Albino Rat
Calendula officinalis / Hypericumperforatum / TCE
This study was conducted to evaluate the hepatoprotective potential effect of Calendula officinalis (C.O) and Hypericum perforatum (H.P) against hepatic inflammation induced by trichloroethylene (TCE) in adult male Spargue Dawely rats. Animals were divided into eleven groups and sub groups(ten rats to each), including a negative control group (untreated) , a positive control group treated orally with corn oil daily for 25 days , a group treated orally with TCE alone (1 g/kg body wt) dissolved in corn oil daily for 25 days, a group treated orally with C.O alone (100 mg/kg body .wt) daily for thirty days, then treated with TCE with a continuous supply of C.O one hour prior to TCE treatment, a group treated orally with C.O alone (250 mg/kg body .wt) daily for thirty days, then treated with TCE with a continuous supply of C.O one hour prior to TCE treatment ,a group treated with C.O (100 mg/kg body .wt) for thirty days after the termination of 25 days of TCE treatment, the last group treated with C.O (250 mg/kg body .wt) for thirty days after the termination of 25 days of TCE treatment. Other animal groups and sub groups treated orally with H.P , a group treated orally with H.P alone (50 mg/kg body .wt) daily for two weeks, then treated with TCE with a continuous supply of H.P one hour prior to TCE treatment, a group treated orally with H.Palone (100 mg/kg body .wt) daily for two weeks days, then treated with TCE with a continuous supply of H.P one hour prior to TCE treatment ,a group treated with H.P (50 mg/kg body .wt) for two weeks after the termination of 25 days of TCE treatment, the last group treated with H.P (100 mg/kg body .wt) for two weeks after the termination of 25 days of TCE treatment. The results indicated that treatment with TCE alone resulted in significant increases in serum liver enzymes ALT and AST combined by a significant increase in serum total and directbilirubin levels, proinflammatory cytokines IL-1β, IL-6 and TNF-α levels as well as increase in liver malondialdehyde (MDA) concomitant with significant decreases in hepatic total antioxidant capacity levels, compared to negative and positive control groups. Co-treatment with either C.O or H.P and TCE resulted in a significant improvement in these parameters and succeeded to restore most of them towards the normal values of the control. Noteworthy, the pretreatment with C.O followed by C.O plus TCE was more effective than when C.O was administered after stopping TCE treatment and the pretreatment with H.P followed by H.P plus TCE was more effective than when H.P was administered after stopping TCE treatment In conclusion, the protective effects of Calendula officinalis (C.O) and Hypericum perforatum (H.P) against TCE -induced liver inflammation may be attributed to their antioxidant and free radical scavenging activities and their higher contents of antioxidant components.
2015
Ph.d
Zagazig
Science