5481
Wessam Elsaeed Mohamed Sharaf-Eldin
"Assessment of the diagnostic value of the expression level of serum miR-145, miR-223 and miR-326 in multiple sclerosis"
Multiple Sclerosis , miRNAs , NMOSD , NPSLE , DRB1*15:01
Background: Multiple sclerosis (MS) is a progressive inflammatory disease that attacks the central nervous system (CNS). It affects primarily young adults representing one of the most incapacitating diseases in this age range. Up to date, no laboratory test or test set can guarantee MS diagnosis. Dysregulation of miRNAs is associated with many diseases, where analyzing the expression of circulating miRNAs may be a promising approach for blood-based diagnosis of MS. Methods: Serum miRNAs were obtained from 37 MS patients and 23 healthy age-matched controls, along with 20 patients with neuromyelitis optica spectrum disorder (NMOSD; n=10) and neuropsychiatric systemic lupus erythematosus (NPSLE; n=10). The expression levels of miR-145, miR-223 and miR-326 were analyzed using real time PCR. RNA was also isolated to investigate key targets that could be of relevance to MS pathophysiology. Moreover, DNA was extracted to study the association of DRB1*15:01 with MS susceptibility. Results: The three investigated miRNAs were found to be upregulated in MS patients compared to healthy controls and their expression profiles showed highly diagnostic potential for distinguishing MS from NPSLE. Combined use of these miRNAs would also be useful for discriminating between MS and NMOSD as well as relapsing-remitting MS (RRMS) and secondary progressive MS (SPMS). Analyzed mRNA targets, SMAD3 and SP1, revealed decreased expression in MS and NMO patients. However, in NPSLE patients, only SMAD3 was downregulated. Frequency of DRB1*15:01 was very low in the enrolled subjects. Conclusion: This study provides evidence that analysis of miRNA expression may be a promising approach for diagnosis and differentiation of MS. However, the frequency of DRB1*15:01, which is highly associated with MS in different ethnic groups, seems to be rare in Egyptian population
2018
Ph.d
Ain Shams
Medical research institute