ANTITUMOR AND ANTIOXIDANT ACTIVITIES OF Solandra grandiflora AND Cassia alata
This study aims to investigate the major constituents of Solandra grandiflora Sw. and Cassia alata (L.) Roxb. leaves. In addition to, evaluate the antitumor and antioxidant activities of the crude methanol extract and its fractions. Phytochemical screening of the crude methanolic extract, determination of total phenolics and total flavonoids of S. grandiflora and C. alata leaves were investigated. Hexane fraction of each plant was saponified into unsap. and sap. (Fatty acids), then analyzed by GC/MS. Fatty acids composition of S. grandiflora leaves comprised a much higher proportion of linolenic, palmitic, linoleic and stearic acids. C. alata leaves were found to comprise a much higher proportion of palmitic, α-linolenic, linoleic and stearic acids. Crude fractions of each plant was fractionated by silica gel and sephadex LH-20 column chromatography and further purified by preparative HPTLC and paper chromatography. Each separated pure compound was identified by 1H-NMR, Mass spectrum and UV. Emodin, aloe-emodin, rhein, kaempferol-3-O-gentiobioside, aloe-emodin-8-O-β-D-glucopyranoside, kaempferol, quercetin, rhein-8-O-β-D-glucopyranoside and emodin-8-O-β-D-glucopyranoside were isolated and identified from C. alata leaves. In addition, scopoletin and aesculin were isolated and identified from S. grandiflora le