5260
Radwa Hosam El-Din Helmy Higazy
Molecular characterization of Tolllike receptors 1 and 6 genes in river buffalo
River buffalo, Toll-like receptor 1, Toll-like receptor 6, polymorphism, protein domains.
Egyptian buffalo are of the river type (Bubalus bubalis bubalis) of water buffalo. Toll-like receptor (TLR) genes play an important role in immune response and defense against invading pathogen. In this study, TLR1 and TLR6 were characterized in river buffalo; Egyptian breed. We are here reporting, for the first time, 22 SNPs in TLR1 and 13 SNPs in TLR6 coding regions. The non-synonymous SNPs (nsSNPs) were 7 (2 were adjacent) and 6, respectively, leading to 6 amino acids (a.a.) substitutions for each. The two genes have no signal peptide. Egyptian buffalo TLR1 had 6 a.a. substitutions: 4 in LRR region, one of which was in low complexity region (LCR) in addition to 2 in TIR domain. All TLR1 a.a. substitutions were found tolerable. In TLR6 six a.a. substitutions were detected: one in transmembrane domain, 3 in LRRs and 2 in TIR domain. All TLR6 a.a. substitutions were tolerable, except TLR6_H713P which was intolerable, possibly affecting protein function and the response to invading pathogens. The protein architecture domains, nucleotide and protein sequences of TLR1 and TLR6 in Egyptian breed were compared with Indian river buffalo breeds, cattle, sheep and goat. Egyptian breed TLR1 has 3 LCR whereas other buffalo breeds, sheep and goat have 2, and cattle have 1 LCR. LCRs are thought to be involved with rapid immune adaptation to the environment of the host among other functions. Egyptian breed TLR6 was mainly different from Nili-Ravi breed. Nili-Ravi breed has 1 LRR less than Egyptian and Murrah breeds in addition to an insertion of 3 nucleotides in its coding region that is present in sheep and goat but not in cattle and other buffalo breeds
2016
M.Sc
Cairo
Science