5540
Jackline Samir Kamal Fahim
Phenotypic and Morecular Detection of oXA_48 gene in Entrobacteriacea
Phenotypic and Morecular Detection ,oXA_48 gene in Entrobacteriacea
Carbapenem resistance among Enterobacteriaceae is an emerging problem worldwide. Several resistance mechanisms have been reported to circurnvent the efiflcacy of carbapenems, and carbapenemases. A large variety of carbapenemases belonging to three molecular classes of Blactamases have been identified in Enterobacteriaceae: the Ambler class A e.g (KPC), class B e.g (NDM and IMP) and class D e.g (OXA 48) B lactamases. The OXA-48 is an active-serine-site enzyme like Ambler class A and class C B-lactamases, differing from class A and C enzymes in amino acid structure and is usually not inhibited by clavulanic acid, tazobactarn, and sulbactam. It was first identified from a carbapenem-resistant Klebsiella pneumoniae isolate in Turkey 2001 and was found to be multidrug resistant and exhibited a high level of resistance to all plactams, including broad-spectrum cephalosporins, cephamycins, monobactams and carbapenems. During this study, 50 specimens were submitted to the Central Microbiology Laboratory of Ain Shams University Hospitals for routine culture and susceptibility testing. Specimens were subjected to identification of isolates by using conventional methods including morphology on MacConkey agar, Gram stained film and biochemical reactions. Antibiotic susceptibility testing for isolates was done by Kirby Bauer disk diffusion method according to CLSI (2014) and they were resistant to carbapenem. Confirmation of carbapenem resistance was done by performing broth microdilution method for MIC detection. Identification of carbapenem resistant classes using carbapenemase detection set by (MastDiagnostics) and temocillin disk was done followed by singleplex real time PCR to detect OXA 48, KPC, NDM, VIM and IMP genes
2016
M.D
Ain Shams
Medicine