5194
Mohamed Ahmed Salem Ibrahim
Comparative Study between Slow Conventional Freezing and Cryoprotectant-Free Vitrification of Human Spermatozoa.
Conventional, Cryopreservation, Freezing, Thawing andVitrification.
The aim of this study is to compare the technology of cryoprotectant-free vitrification of human spermatozoa in large volume, to conventional freezing protocol as regards post-thawing motility and vitality. Semen samples were collected from male subjects, accepting to sign a consent, and then semen analysis was done. According to results, 20 samples were included, classified into two groups; (Group 1) normal, mild or moderate oligozoospermia and (Group 2) severe oligozoospermia. Samples were prepared then splitted into 3 aliquots; (Aliquot 1) control group (fresh ejaculate), (Aliquot 2) spermatozoa cryopreserved by slow conventional freezing with glycerol-containing medium and (Aliquot 3) spermatozoa vitrified in 0.3 ml insemination "French" straws in culture with 0.25 M sucrose containing medium.Motility and vitality (Hypo-osmotic swelling test) of vitrified spermatozoa displayed statistically significant lower levels as compared to conventional freezing.
2016
M.Sc
Cairo
Medicine