5314
Eman Nasr Mohammed Hosny
Protective and Therapeutic Role of Curcumin and
Caffeine in Experimental Model of Parkinson’s Disease
in Rats
curcumin, caffeine, Parkinson’s Disease, Dopamine, midbrain. striafum
The present study was carried out to evaluate the protective and therapeutic effect of curcumin, caffeine and their combination in rat model of Parkinson’s disease (PD). The study included 110 adult male albino rats, divided into eleven groups (10 each): control group injected daily with the vehicle (1 ml/kg, i.p.), CUR group received daily oral administration of curcumin, CAF group injected daily i.p. with caffeine, CUR+CAF group received daily oral administration of curcumin and i.p. injection of caffeine, ROT group represented the rat model of PD that was induced by the daily injection with rotenone (1.5 mg/kg i.p for 45 days), CUR/Pr is the rat model of PD protected with curcumin, CAF/Pr is the rat model of PD protected with caffeine, CUR+CAF/Pr is the rat model of PD protected with curcumin and caffeine combination, CUR/Tr group includes the rat model of PD treated with curcumin, CAF/Tr group includes the rat model of PD treated with caffeine and CUR+CAF/Tr group includes the rat model of PD treated with curcumin/caffeine combination. The used curcumin dose was 80 mg/kg b.w. and the used caffeine dose was 30 mg/kg b.w. A decrease in locomotor activity was observed in rotenone-intoxicated rats. In addition, a state of oxidative and nitrosative stress, neurodegeneration and inhibition in acetylcholinesterase (AchE) and Na+/K+-ATPase activities were detected in midbrain and striatum of rat model of PD. This was associated with an increase in tumor necrosis factor-α (TNF-α) level and depletion in dopamine (DA), norepinephrine (NE) and serotonine (5-HT) levels in the two studied brain regions. The protection and treatment of rat model of PD withcurcumin and/or caffeine ameliorated the oxidative stress and the changes in TNF-α level, AchE and Na+/K+-ATPase activities induced by rotenone. This was associated with an improvement in the histopathological changes induced in the two brain regions of PD model. Also, both protection and treatment with curcumin and/or caffeine restored the depletion of midbrain and striatal DA induced by rotenone and prevented the decline in motor activities. Protection and treatment with curcumin and/or caffeine restored the decrease in brain NE and 5-HT levels. However, protection and treatment with caffeine alone failed to normalize the depletion in midbrain and striatal 5-HT induced by rotenone in. In conclusion, the present study showed that both curcumin and caffeine either given alone or in combination offered a significant neuroprotection and treatment against biochemical, neurochemical, histopathological and behavioral changes in rotenoneinduced rat model of PD.
2016
Ph.d
Ain Shams
Science