5165
Shimaa Farag Hemida Hussien
Protective Effect of some Bee Products against Gamma Irradiation in Rats.
Gamma Irradiation, Honey, Propolis, Total Phenol, Total Flavonoid, Antioxidants, Minerals, Biophysical and Biochemical analysis.
This study was designed to investigate antioxidant activity of honey and propolis in vitro through colorimetric determination of total phenol, total flavonoid and free radical scavenging activity, In addition to, spectroscopic evaluation of such compounds using UV-Visible, FTIR. Moreover, HPLC was used for identification of such bioactive compounds in addition to Vitamin E, C and β- carotene. While, the concentration of some minerals was measured by atomic absorption spectroscopy. Also, in vivo study in male rats exposed to fractionated dose gamma irradiation (1 Gy every day up to 5 Gy total doses). Honey, propolis and their combination administrated at dose 250, 90 and (250+90) mg.kg-1day-1 respectively. The serum levels of (ALT) and (AST), urea, uric acid, creatinine, total protein, albumin and total antioxidant capacity were estimated. Moreover, hemoglobin was investigated through UV absorption spectrum and dielectric measurements. While, RBCs membrane was investigated through osmotic fragility and blood film.
2016
Ph.d
Cairo
Science