5065
Engie Mohamad Moustafa Safwat Niazy
Preparation of Mineralized Nanocellulose Hydrogel from Some Local Agricultural Residues and Assessment of their Viability for Use in Osseous Regeneration (In Vitro Study).
Nanoellulose hydrogel - bone graft – rice straw –calcium phosphate- bagasse
The aim of this study was to prepare mineralized nanocellulose hydrogel from recycled agricultural wastes, characterization of the prepared nanocellulose hydrogel using Fourier transform infrared spectroscopy. (FTIR), Transmission electron microscope. (TEM), Scanning electron microscope (SEM),X-ray diffraction (XRD) and in vitro biocompatibility evaluation of the prepared cellulose hydrogel. An injectble hydrogel bone graft for use in the construction of bone tissue was prepared using nanocellulose hydrogel isolated from rice straw. Bleached cellulose pulp was first prepared from rice straw and bagasse by pulping using 15% sodium hydroxide (based on weight of rice straw) followed by bleaching using sodium chlorite/acetic acid mixture. Then the bleached pulp was oxidized using 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)/chlorite method, purified by repeated washing with distilled water, and finally subjected to high shear mixer to prepare nanocellulose hydrogel.Mineralization of the prepared nanocellulose hydrogel was done through in situ mineralization of the hydrogel, incorporation of biphasic calcium phosphate (prepared by two methods) and incorporation of amorphous calcium phosphate The injectable consistency of the obtained nanocellulose hyrogel was adjusted through calibrating the water content to the solid content in the hydrogel so that every 1gram dry weight nanocellulose fibers is proportioned to 15 gram water. The biocompatibility of the prepared injectable bone graft hydrogel extracted from rice straw and bagasse were inspected in the vicinity of osteoblasts in vitroand the ability of the gel to induce bone formation was confirmed through two tests: the alkaline phosphatase assay and the Von Kossa test Data were collected and statistically analyzed using One way ANOVA recorded statistically significant (P<0.05) Results revealed that, nanocellulose hydrogel extracted from rice straw withoutmineralization produced a nontoxic response on osteoblasts in vitro and had the ability to induce bone formation.
2015
Ph.d
Cairo
Dental Medicine