4971
SORAYA ADEL HASSAN
STUDIES ON CAROTENOIDS IN SOME EGYPTIAII FOODS AND ITS EFFECT ON DEVELOPING THE COMPLICATIONS OF DIABETES MELLITUS USING RATS
Diabetes Mellitus / Tomato, / lyceopene / HbA1C, / AngiotensinConverting enzyme
The present investigation included two parts, the first one aimed to quantifr lycopene s a major carotenoid concentration among some Egyptian foodstuffs in addition to study the effect of lycopene as antioxidant on angiotensin converting enzyme (ACE). The second part concerned with a survey to investigate nutritional and health status of the pregnant women and evaluate their dietary practices during pregnancy with special consideration to lycopene intake. Results in part I showed that, the content of fresh tomatoes accounted to 2.62 mg/100g of fresh weight. However, the content of tomato paste samples in different concentrations (20, 22e25%) was 9.6, 10.2 &15 mg/I00 g without significant difference between them. While,Lycopene content of ketchup accounted 12.95 mgllO9 g. An experimental trail included 28 adult Wister rats categorized into four equal groups (n : 7).The first group (C) was negative control group, the second one (D) was diabetic group(positive control) , the third (L) lycopene group and the fourth (DL) diabetic- Lycopene group. Diabetes was induced in rats by streptozotocin (45mglkg of the body weight). Daily lycopene dose (10 mglkg) was orally given for 5 weeks by stomach tube. Results revealed that, the percentage of hemoglobin Alc in D group was significantly (p<0.001)highercompared to rats in control group. However, the differences between these percentage in C and L groups were insignificantly. The percent of hemoglobin Alc was significantly decreased (p < 0.05) in DL group compared to diabetic rats @ group), but it did not reach to those found in control rats. Group D rats had the highest level and elevated ACE activity (p < 0.05) over those of control (C) rats group. Treatments of diabetic rats with lycopene significantly decreased ACE activity (p < 0.05) in the DL group ,but the enzyme activity was significantly higher than that in the negative control group (p < 0.05). Although, there was insignificant difference between rats in the two groups (L&DL) in ACE activity. Concerning second part, a 24-Hours dietary recall was conducted using a special design questionnaire for a sample of 100 pregnant women were randomly chosen from hospital, oftwo cities, Cairo and El Mansoura, 50 pregnant women in each area . Results showed that, most of the pregnant women were less than 30 years old. High percentage of them (40 and 54 %) followed up by doctors starting from the first trimester until delivery in Cairo and El- Mansour4, respectively. High percentages of pregnant women attended education programs , being 72& 66% in Cairo and El-Mansoura, respectively. High significant differences between the study groups (p<0.01) in their mean intakes of daily total protein, animal protein and vitamin C. Close to one third of the sample (26&32, and30&24%o)of pregnant women in both groups their intakes were above 100% from DRI of total protein and vitamin C, respectively. There were iniignificant differences between the two groups under study (p>0.05) in their means of Hb, blood glucose, serum lycopene levels ,lipid prophile andkidney functions.
2015
M.Sc
Cairo
Agriculture