5319
AMR MOHAMMED ABO-ELHAMD
ANTIOXIDANT AND ANTIHEPATOTOXIC ACTIVITIES OF SOME EGYPTIAN MEDICINAL PLANTS
Calliandra haematocephala (Hassk.), Breynia nivosa, Flavonoids, Sterols, Terpene, Antihepatotoxic and Antioxidant
This study aims to investigate the major constituents of Calliandra haematocephala (Hassk.) and Breynia nivosa leaves. In addition, the hepatoprotective and antioxidant activities of the total alcoholic extract against carbon tetrachloride (CCl4) -induced liver damage in vivo were studied. Phytochemical screening of the various fractions of B. nivosa and C. haematocephala leaves revealed the presence of terpenoids, phytosterols, alkaloids, fixed oils, phenolics, flavonoids, tannins and saponins. Hexane fraction of C. haematocephala and B. nivosa leaves were fractionated by column chromatography and further purified by preparative HPTLC. From hexane fraction of C. haematocephala leaves lupeol and five sterols were isolated whereas, lupeol, lupeol acetate and β-sitosterol were isolated from hexane fraction of B. nivosa leaves. Ethyl acetate fraction of C. haematocephala and B. nivosa leaves were fractionated by column chromatography and Sephadex LH-20 column and further purified by preparative HPTLC and on paper chromatography Whatmann No 3. Six flavonoids were isolated and identified from C. haematocephala leaves. Two flavonoids and one phenolic acid were isolated and identified from B. nivosa leaves. Fatty acids composition of B. nivosa leaves comprised a much higher proportion of palmitic and linolenic acid . Unusual fatty acids 11- Octadecenoic acid and 10- Octadecynoic acid were detected in appreciable amounts. C. haematocephala leaves was found to comprise a much higher proportion of palmitic, linolenic acid, oleic, and linoleic acids. In vivo, the hepatoprotective and antioxidant activities of methanolic extract against CCl4-induced liver injury was evaluated in rats based on the analysis of biochemical parameters and histopathological. In both protective and therapeutic experimental CCl4 treated animals with alcoholic extract at 100 and 200 mg/kg bw. of C. haematocephala and B. nivosa leaves significantly (p<0.05) decreased AST, ALT, γ-GT and bilirubin levels and increased albumin level, decreased serum creatinine and urea level. The extract at both doses exhibited significant (P < 0.05) reduction in the level of MDA. Oral administration of the extracts at 100 and 200 mg/kg bw. in rats treated with CCl4 showed recovery of the activities of antioxidant enzymes (CAT and SOD) and GSH and elevation of the concentrations comparing to CCl4 induced hepatotoxicity in rats. The high dose 200 mg/kg bw. was more significantly effective than low dose 100 mg/kg as compared with CCl4 treated rats and the results were confirmed byhistopathological studies.
2016
Ph.d
Cairo
Agriculture