5546
Eman Omar Abdel Fattah Mohammed Fathalla
Studies on In Vitro Production and Biological Activity of Bioactive Substances from Cleome Droserifolia Endemic in Egypt
Cleome Droserifolia , In Vitro , HPLC, antidiabetic
Herbal medicines are promising choice over synthetic drugs, Cleome droserifolia medicinal herb belongs to Family Cleomaceae. The dried herb of C. droserifolia, locally named in Egypt Samwah, Reeh-El-Bard in which used as hypoglycemic plant by herbalists. In vitro cultures could be a possible alternative to analyze crude extract for pharmacological activities rather than collecting and destroying possibly endangered plants and obtaining important phytochemicals in vitro. In this study, callus cultures were produced using hypocotyl and root explants of C. droserifolia using different combinations of growth regulators, The maximum of callus percentage was observed with root explants cultured on MS media supplemented with 0.5 mg/l a-naphthaleneacetic acid )NAA( + 1 mg/l benzyl adenine)BA( and methanol extract of callus was evaluated for their antidiabetic potential. Diabetes mellitus was induced by injection of single intrapretoneal dose of streptozotocin (STZ) (60 mg/Kg body weight), The crude methanolic extract prepared from the callus of C.droserifolia hypocotyl cultured on media 5 contains 1 mg/l BA (200 mg/kg) was orally administered to STZ-induced diabetic rats once daily for 4 weeks, results demonstrated that, restored serum glucose levels along with improved insulin levels of diabetic rats. Decreased serum aspartate aminotransferase (AST) and alanine aminotransferases (ALT) activites and malondialdehyde (MDA) level while increament of total Antioxidant Capacity (TAC), glutathione (GSH), serum protein, thyroid hormones (T3 and T4) levels were also increased after administration of plant callus extract. The effect of plant crude callus extract on liver and pancreatic morphology were examined. Furthermore, HPLC chromatograms of crude methanolic callus extract showed the presence of two flavonoids (quercetin and apigenin) which may explain the effect of antidiabetic and antioxidant properties.
2019
M.Sc
Cairo
Science