5652
Wedian El-Sayed Mohamed Ashour
A Pharmacognostical study of Saraca indica L. and Peltophorum africanum Sond.Family Fabaceae
Peltophorum africanum Sond & Saraca indica L, UPLC/MS analysis,Antidiabetic , anti Alzheimer,Nanotechnology
Peltophorum africanum Sond. and Saraca indica L. are plants belonging to family Fabaceae. GC/MS analysis of the volatile constituents of Saraca indica leaves revealed the identification of 19 compounds and 32 compounds of P. africanum. .Analysis of the unsaponifiable fraction of the petroleum ether extract of Peltophorum africanum leaves resulted in the identification of 20 compounds . Analysis of the fatty acids of P. africanum leaves resulted in the identification of 14 compounds. Analysis of the fatty acids methyl ester of the petroleum ether extract of Saraca indica leaves resulted in the identification of of 26 compounds. Crude leaf extracts of P.africanum Sond. and S.indica L.were analyzed via reversed phase UPLC/PDA/ESI-MS in negative ionization mode Representative UPLC-MS chromatograms of P. africanum and S. indica crude extracts resulted in a total of 46 chromatographic peaks belonging to various metabolite classes were derived. UV, 1H-NMR,13C -NMR, MS spectroscopy) to identify their chemical structures . Nine compounds are isolated and identified for the first time from the leaves of P. africanum Sond. in this study and Six compounds are isolated and identified from the leaves of S.indica L . Bioactivity studies were carried out on the total ethanol and successive extracts of Peltophorum africanum Sond. and Saraca indica L . Leaves.The results of the antioxidant activity by three different methods revealed that the % scavinging activities. Investigation of the antioxidant activity of Saraca indica L. by DPPH assay revealed that there is an increase in % scavenging activity as the conc. of the plant extract increase (0.01, 0.05 g %). The TEE was the most potant extract at two selected dose levels followed by EtOAc, CHCl3 and pet.ether .Cytotoxic activity of both plants give high activity .Acetylcholinesterase Inhibitory Activity of the TEE of P.africanum , S.indica & Tacrin as standard drug were tested. The inhibition of α-amylase on the previous extract, appears to be dose dependent. Therefore, in vivo biological evaluation was carried out selecting EtOAc , TEE and PEE and for each of PA and SI, as the most potent extracts with proposed antidiabetic activity. Estimation of the antimicrobialactivity of AgNPs which revealed goo activity
2020
Ph.d
Cairo
Pharmacy