5535
Gomaa Said Ramadan Gad
Molecular Markers As A Tool For Selection In Local Egyptian Chickens
Cairo B-2, Microsatellite Markers, Heterozygosity, Polymorphic Information Content, Broiler Breeders, Body Weight, SRAP.
The present study was done to determine the effects of selection, for twelve generations for increased 6-week body weight, on the body weight and carcass characteristics of Cairo B-2 line in comparison with the Random Breed Control line. (RBC). The result indicated that Cairo B-2 line was significantly heavier 35.4% for males and 33.2% for females than RBC line. The slaughter trial indicated that Cairo B-2 line had significantly higher carcass, breast meat, leg meat, and wings with bones weights than RBC line at 6 weeks of age. Males, of both lines, had significantly higher carcass, breast meat, leg meat, and wings with bones weights compared to the females. The phenotypic correlations between LBW at 6 weeks of age and all other traits studied in comparison to RBC line, for 11 generation. Also sixteen microsatellite markers, related to body weights and carcass part weights were investigated. The average number of alleles for these sixteen microsatellite loci for Cairo B-2 was 9.5 compared to 8.1 alleles for RBC line. The average heterozygosity value for the microsatellite loci were 0.726 ranging across loci from 0.620 to 0.820 for Cairo B-2 line. The average heterozygosity was 0.743 ranging between 0.625 and 0.815 for RBC line. The polymorphism information content among these loci ranged between 0.555 to 0.833 with a general mean of 0.744 for Cairo B-2. It ranged from 0.555 to 0.845 with a general mean of 0.762 for RBC line. The correlation coefficients between the allelic numbers for the studied alleles and 6-week LBW, carcass, breast meat and other investigated traits were generally significant (P≤ 0.05) and positive. It was thus suggested to use these microsatellite markers, in the future for Cairo B-2 line selection program. This may speed the response to selection.
2019
Ph.d
Cairo
Agriculture