5297
Mona Mohamed Hassan Soliman
Rapid immunological assays for detection of
aflatoxin B1 in animal feed and its products
Aflatoxin B1, HPLC, Polyclonal antibody, ELISA, TLC, Animal feed, Meat,
Meat products
Fungi and their toxins are extensively widespread as those fungi are ubiquitous in nature. Aflatoxin B1, a fungal secondary metabolite excreted mainly by toxigenic Aspergillus flavus, considered as the most dangerous naturally occurring toxin, has carcinogenic effect on both human and animals. Aflatoxin B1 was successfully produced from local toxigenic Aspergillus flavus strain on YES medium and its amount was determined using HPLC. Aflatoxin B1 is a toxic fungal metabolite of low molecular weight, and hence is devoid of antigenicity. Those toxins also lack a reactive group for coupling with a macromolecule carrier for antibody production. Therefore, the main goal of this study was to produce polyclonal antibody (IgG) against aflatoxin B1 through immunization of New Zealand white rabbits by the prepared antigen (AFB1-BSA Conjugate). The produced polyclonal antibodies were used in the production of a diagnostic product that was tested against spiked and naturally contaminated samples. Spiked samples were tested using both sandwich and competitive ELISA. In case of sandwich ELISA, the recovery % obtained from finished cattle feed samples ranged from 80.3% and 96% while in meat samples AFB1 ranged from 78.1% and 93%. For competitive ELISA, the mean recovery % of AFB1 obtained from finished cattle feed samples ranged from 88.7% and 97% , while in meat samples it ranged from 89.6% and 103%. Using competitive ELISA, The produced product gave promising results and was efficient in detecting AFB1 in the examined naturally contaminated samples. In conclusion, the diagnostic product produced in the present study is simple and economic. ELISA procedure that can be used to screen aflatoxin B1 in variety of samples (animal feed samples, meat and meat products). Thus, it is possible to screen large number of samplessimply and inexpensively for the presence of aflatoxin B1
2017
Ph.d
Cairo
Veterinary Medicine