5465
SOHA AHMED ABD EL-LATIFE HUSSIEN MURAD
CHEMICAL CHARACTERIZATION AND BIOLOGICAL EVALUATION OF ALGAL PEPTIDES
Spirulina platensis, Scenedesmus obliquus, Algal Peptides, Antioxidant activities, Antiviral activities, cytotoxic activities
This study was conducted to study the production of peptides from Spirulina plarensis and Scenedesmus obliquus, their biological activities as antioxidant, antiviral, and anticancer were also investigated. Protein was extracted from Spirulina platensis by three different extraction methods (Sp1, Sp2 and Sp4). Protein extracts and hydrolysates were assessed by gel electrophoresis (SDS–PAGE) of samples and marker. Algae cells and protein extracts were rich in Arg, Lys, Asn, Ala and His. Antioxidant activity of protein hydrolysates from Spirulina was investigated. Protein hydrolyzed by trypsin (Sp2Try) and (Sp4Try) showed the highest antioxidant activity against 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical-scavenging (32.27% and 30.11%,respectively) and on 2, 2'-azinobis 3-ethyl- Benzothiazoline-6-sulphonate(ABTS) radical (56.39% and 34.26%, respectively) at sample concentration (150 μg/ml). Protein was extracted from S. obliquus by three different extraction methods. Protein extracts and hydrolysates were assessed from stained gels following SDS–PAGE of samples. Antioxidant activity of protein hydrolysates was investigated. Protein hydrolyzed by papain (Sd1pa) and protein hydrolyzed by trypsin (Sd2Try) induced highest antioxidant activity based on 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical- scavenging (41.41% and 40.62%) respectively, and on 2,20-azinobis 3-ethyl- benzothiazoline-6-sulphonate (ABTS) radical (87.03% and 45.12%) respectively, at 150 mg/ml. The inhibitory effect and mode of action of protein hydrolysates were evaluated against Coxsackie B3 virus (CVB3). Protein hydrolyzed by papain (Sd2pa) and protein hydrolyzed by pepsin (Sd1pep) at 100 mg/ml exhibited antiviral activity (66.2% and 57.6%, respectively), against (CVB3) from all protein hydrolysates. S. platensis and S. obliquus protein hydrolysates can be used as nutritional food with antioxidant properties. S. obliquus protein hydrolysates have a potential as antioxidative neutraceutical ingredients and a potential therapeutic agent against CVB3. The sequence of peptides obtained from Spirulina and Scenedesmus were determined by using MALDI-Triple TOF method
2018
Ph.d
Cairo
Agriculture