5634
Neama Abdelmoneim Abdelaal Abdalla
MICROPROPAGATION OF JERUSALEM ARTICHOKE (Helianthus Tuberosus L.)
PLANT
Helianthus tuberosus, micropropagation, callus cultures, regeneration, molecular characterization, RAPD, ISSR and SCoT
This study was carried out on three cultivars of Jerusalem artichoke (i.e., Alba, Fuza and Balady). The main target was to establish a cost effective and an efficient in vitro propagation protocol of J. artichoke by tissue culture technique for large scale production. The tubers of J. artichoke were released of dormancy by using 400 mg L-1 gibberellic acid. Aseptic cultures of stem nodes were successfully initiated on ½ MS + 62.5 mg L-1 cefotaxime. The best results for shoots multiplication were observed on MS + 1 mg L-1 BA + 0.1 mg L-1 NAA + 50 mg L-1 nano-selenium. In vitro shootlets were efficiently rooted on ½ MS + 2 mg L-1 IBA + 0.1 mg L-1 NAA + 0.5 mg L-1 kin. In vitro derived plantlets were well acclimatized on a mixture of peat moss, sand (1:1). In addition, callus cultures of Balady, Fuza and Alba cultivars were successfully established from stem explants on MS supplemented with 1 mg L-1 BA + 2 mg L-1 NAA. Callus cultures could be used for inulin production.
2020
Ph.d
Ain Shams
Agriculture