Biotechnological studies on L-methioninase from some Streptomyces isolates.
L-methioninase. Streptomyces,
The greater dependency of cancer cells on L-methionine for growth compared to normal cells indicates that depletion of L-methionine using L-methioninase is effective for cancer therapy. Accordingly, the exploitation of L-methioninase as a promising enzyme for anticancer therapy necessitates the search for new isolates producing L-methioninase. Therefore, the current study focused on screening of different Streptomyces isolates (locally isolated from soil) for their ability to produce L-methioninase (the subject of this thesis). Optimization of cultural and nutritional conditions required for highest enzyme production by the most promising Streptomyces isolates was studied. Furthermore, immobilization of Streptomyces cells and its effect on L-methioninase production was also estimated. Moreover, purification and characterization of L-methioninase from the supernatant of the selected Streptomyces isolate were investigated. In addition, the antitumor; antioxidant and antiviralactivity of the purified enzyme was evaluated.