5566
Reda Ismail Sayed Ahmed El-sayad
Genetic And Molecular Studies On The Effect Of Paracetamol And The Protective Role Of Moringa As A Natural Antioxidant On Mice.
Paracetamol, Moringa oleifera , Molecular Studies
In the present investigation, the genotoxic effect of antipyretic – analgesic drug, Paracetamol was evaluated in male mice. Oral administration of Paracetamol at two tested doses 500 & 2000 mg/kg b. wt. either as a single dose or seven consecutive doses within 24 h intervals showed its genotoxic effect. Determination the possible protective role of oral administration of Moringa oleifera leaf extract as a natural antioxidant at 200 mg/kg b. wt. for 10 consecutive doses within 24 h interval either before or after Paracetamol administration, applying different experiments as studying the induction of chromosomal aberrations in bone marrow cells, sperm abnormality test in morphology, DNA fragmentation test in liver tissue cells, performing electrophoretic pattern of DNA extracted from liver and determination of reduced glutathione (GSH) content in liver tissue homogenate. Molecular biology results confirmed the results obtained from cytogenetic & biochemical study as Paracetamol has a genotoxic effect on increasing the incidence of DNA fragmentation & decreasing reduced glutathione content (GSH) compared to control group. Pre-treatment with Moringa oleifera leaf extract is better than post-treatment against genotoxicity of Paracetamol.
2018
Ph.d
Ain Shams
Science