5354
MOHAMAD MAGHAWRY IBRAHIM ABOELENIN
CHARACTERIZATION OF SOME GENES
RELATED TO FERTILITY IN
EGYPTIAN BUFFALOES
Egyptian river buffaloes, LEP, DGAT1, BMP4, GHR, TNFα,
PCR-RFLP, SNP, sequencing, cis-acting splicing regulatory
elements, protein function, protein stability, mRNA
secondary structure and translation elongation rate
River buffalo (Bubalus bubalis) is an important economic resource to millions of farmers in Egypt and several developing countries. But the reproductive performance of river buffalo females is poor as a result of inherent reproductive problems such as retardation of ovarian cycle reactivation post-partum, long calving interval, the higher number of services per conception and a high rate of pregnancy failure due to the high levels of embryonic and fetal mortalities. Thus, the present investigation studied the polymorphism in LEP, DGAT1, BMP4, GHR and TNFα genes which related to fertility in 81 female Egyptian river buffalo. The PCR-RFLP pattern of these genes using the restriction enzyme Eco91I, EaeI, HinfI, SspI and RsaI , respectively showed that all the animals had monomorphic pattern in all studied genes consists of CC, AA-AA, GG, TT and CC genotypes , respectively. The sequencing results of these genes showed 7 single nucleotide polymorphisms (SNPs) among the Egyptian buffaloes and 46 SNPs were detected between Egyptian buffaloes and other buffalo breeds records in GenBank. Finally, in silico analysis was performed to study the effects of the detected SNPs on the cis-acting splicing regulatory elements, protein function, protein stability, mRNA secondarystructure and translation elongation rate. The amplified region in the buffalo genes was compared with its orthologous gene in cattle, sheep, goat, human and mouse on the DNA and translated amino acids levels. The discovered SNPs could be used as marker assisted selection (MAS) tools in breeding programs aim to improve the reproductive performance in buffalo females.
2017
Ph.d
Ain Shams
Agriculture