5182
Samira Ali Hassan Osman
Genetic Studies on some Hordeum vulgare Varieties
Hordeum vulgare L, Salt stress, Agro-morphological traits, SDS-PAGE, proline, Molecular markers, BADH-1 gene expression, sequencing.
The present investigation was conducted to study the phenetic and the phylogenetic relationships between ten Egyptian Hordeum vulgare L varieties depending on agro-morphological criteria, molecular genetic markers (RAPD-PCR, ISSR-PCR and microsatellite SSR-PCR) and protein banding patterns electrophoresis. This study also determined the viable marker which has potential power in discrimination and identification of cultivars and landraces. The results of agromorphological traits measurement reflected that Giza 2000 and Wadi Sedr gave good vegetative growth and yield productivity, in contrast Giza 129 gave poor vegetative growth and yield productivity. In RAPD analyses using 22 random primers, we recorded a total number of bands 316, from which 231 (41.77%) were polymorphic while in ISSR analyses using 10 primers, the total bands (159) were detected, among which 98 bands (62.02%) were polymorphic, also SSR analyses using 7 primers showed a total of 15 bands, among which 8 bands (61.22%) werepolymorphic. This indicated that ISSR was better than RAPD to detect genetic diversity among the barley varieties. The genetic distance tree was produced by UPGMA based on agro-morphological data stated the pedigree of studied cultivars. However in the genetic distance tree based on molecular markers (RAPD, ISSR and SSR) and analysis of combined data and proteins banding patterns indicated that the genetic relationships among studied cultivars were not in full agreement with the pedigree.
2016
Ph.d
Ain Shams
Science