5661
Nadia Samy Mahmoud Ibrahim
Evaluation of the efficacy of bone marrow
derived- mesenchymal stem cells co-cultured
with nanomaterials in the management of
osteoporosis in rat model
Osteoporosis, Gold,Hydroxyapatite, Chitosan,
Osteoblasts, Bone marrow-derived mesenchymal stem cells,Rats
Aim: This study was designed to elucidate the osteoinductive potential of some selected nanostructures; Gold (Au-NPs), Gold/hydroxyapatite (Au/HA-NPs), Chitosan/hydroxyapatite (C/HA-NPs) Hydroxyapatite (HA-NPs), and Chitosan (C-NPs) on bone marrow- derived mesenchymal stem cells (BM-MSCs) in vitro. This study was further extended to evaluate the therapeutic efficacy of osteoblasts, resulted from culturing BM-MSCs in either osteogenic medium (OS) alone or OS supplemented with one of the chosen nanomaterials, to reverse osteoporosis in ovariectomized rat. Material and Methods: The selected nanomaterials were characterized using transmission electron microscope and Zetasizer. MSCs were harvested from bone marrow of male rat femur bones and their identity was validated by morphology, flow cytometry and multi-potency capacity. The effect of the selected nanomaterials on the viability, osteogenic differentiation and bonematrix mineralization of BM-MSCs was assessed using MTT assay, osteoblast-related gene expression analysis and alizarin red S assay, respectively. The in vivo study was initiated by dividing sixty-four female rats into 8 groups; (1) negative control, (2) untreated ovariectomized rats, whereas groups (3),(4) ,(5),(6),(7) and(8) represented ovariectomized rats treated with osteoblasts generated from culturing BM-MSCs in either OS combined with Au-NPs, Au/HA-NPs, C/HA-NPs, HA-NPs or C-NPs or OS alone, respectively.
2019
Ph.d
Ain Shams
Science