5131
Neama Ibrahim Ali Abo-Bakr Ali
GENETIC POLYMORPHISM STUDY OF SOME QUANTITATIVE TRAIT GENES IN EGYPTIAN BUFFALO AND CATTLE
genetic polymorphism, ABCG2, OLR1, PPARGC1A, SCD1, PCRRFLP, buffalo, cattle, milk
Four positional and functional candidate genes for milk yield and milk composition (fat, proteins) were typed in males and females of Egyptian cattle and buffalo. The studied genes were ABCG2, OLR1, SCD and PPARGC1A (3 positions). Blood samples were collected from 81 animals, 43 buffalo (23 male, 20 female) and 38 cattle (18 male, 20 female). DNA was extracted and polymerase chain reactions were performed using specific primers for each gene under study. Finally, PCR products were characterized using RFLP methodology after cutting the PCR products with specific restriction enzyme to each gene under study. The genotyping results in cattle showed that the OLR1, SCD1 and PPARGC1A (P616l; T19C) were polymorphic and show two alleles with three genotypes. While, the PPARGC1A (A968C) shows two allele with only two genotypes (AA, AC), in contrast ABCG2 gene shows only one allele A with the genotype AA. Genotyping results in buffalo also proved the absence of polymorphism in the ABCG2 and PPARGC1A (T19C, A968C), while the rest of the studied genes showed different degrees of polymorphism. This study proves the possibility of using the genotyped genes except ABCG2 as functional candidate genes for milk production and composition traits in Egyptian cattle, while the genes OLR1, SCD1 andPPARGC1A (P616l) are the only genes can be used in buffalo genetic improvement
2015
Ph.d
Ain Shams
Science