5647
Amal Gouda Hussien Abd El-Hameed
Biochemical Studies on the Effect of Ulmus Pumila Leaves Extract on Chemically-Induced Breast Cancer in Rats
Ulmus Pumila, Breast Cancer
Breast cancer is the most prevalent cancer and foremost global public health problem. There are little drugs to inhibit cancer metastasis or prevent its angiogenesis, current medications are as yet insufficient to generally treat this disease due to adverse side effects drug resistance and non-specificity, and therefore, there is a constant need for the development of novel and improved chemotherapeutic agents for the prevention and treatment of cancer. Therefore, the object of the present study was to evaluate the chemopreventive effect of Ulmus Pumila leaves extract on breast tumorigenesis induced in experimental animals by N-methyl-N-nitrosourea (MNU), in an attempt to inhibit, reverse or restrict the development of breast cancer and inhibit its metastasis and angiogenesis. A total of ninety female Wistar rats were divided into six groups; normal control group, UPME-treated group: rats were orally treated daily with (1/10 of the LD50 of UPME, 141.6 mg/kg) for four consecutive weeks, tumor group: breast cancer was induced in female rats by the intraperitoneal (i.p.) injection of two doses of freshly prepared MNU at a dose of 50 mg/kg bw, prophylactic group: rats were orally pretreated daily for four consecutive weeks with 1/10 of the LD50 of UPME before induction of breast cancer, protective group: rats were orally treated daily with 1/10 of the LD50 of UPME starting with the first (i.p.) injection of MNU and continued till the end of theexperiment (25 weeks) and therapeutic group: rats were orally treated daily for four consecutive weeks (starting from the age of 25 weeks) with 1/10 of the LD50 of UPME after induction of breast cancer. Obtained results revealed that UPME treatment significantlydecreased liver enzymes (ALT, AST and ALP) activities, kidney function (creatinine and urea levels), uPA, HPA, bFGF, Bcl-2 and Cox-2 levels, on the other hand TAC level was increased, as compared to tumor group. This improvement in biochemical results were also supported with morphological changes, as well as histopathological impairment was minimalized in UPME treated groups. The ameliorative effect of UPME in therapeutic group was more effective than that in protective and prophylactic groups which indicated that UPME had chemotherapeutic potential on breast cancer induced rats. This indicated that the present extract has the capacity of inhibiting inflammation, halting angiogenesis, stimulating apoptosis, prohibiting tumor growth, and arresting matrix degradation and subsequently the tumor invasiveness and metastasis with improvement in the histopathological alterations. Furthermore, the improvement in therapeutic group was more pronounced than that in, protective and prophylactic groups, indicating that UPME may act as a therapeutic agent more than being a prophylactic agent. In conclusion UPME could be developedas a promising chemopreventive agent for breast cancer through normalizing the biochemical parameters and improving the morphological and histological investigations, but this vital extract could have more therapeutic action than prophylactic action.
2020
Ph.d
Ain Shams
Science