5646
Eman Mohamed Shehata Ragab Abdellah
Biochemical studies on oil feedstock produced from
oleaginous fungi for biodiesel production and
evaluation of the residual biomass as animal feed
biodiesel, oleaginous fungi, animal feed
Oleaginous fungi have recently gained increasing attention among different microorganisms due to their abilities of lipid production for biofuel preparation. In the present study, a locally isolated fungus E45, identified genetically as Aspergillus sp. strain EM2018 MK377325, was found to produce 25.2 % total lipid of its DCW. Optimization of culture conditions was performed and lipid accumulation was increased about 2.4 folds (from 25.2 % to 60.1 % of DCW) when the fungus grown for seven days in a potato dextrose (50 g/L) liquid medium of pH 5.0, incubation temperature at 30 °C and inoculum size of 2 × 106 spore/mL. Supplementation of the medium with yeast extract and NaNO3 at a concentration of 0.05 % as organic and inorganic nitrogen sources, respectively, further increased lipid production (53.3 % lipid/dry biomass). Gas chromatographic analysis of fungal lipid revealed the presence of saturated (mainly palmitic acid C16:0 (33 %) and lignoceric acid C24:0 (15 %)) and unsaturated fatty acids with different proportions (mainly linoleic acid C18:2 (24.4 %), oleica cid C18:1 (14 %) and arachidonic C20:4 (7.4 %). These findings suggest this new oleaginous fungus as a promising feedstock for various industrial applications and biodiesel preparation.
2020
Ph.d
Ain Shams
Science