5611
Noha said hussein said
Lipidomic study on the effect of eclipta prostrata and bouhinia retuse on expevimeatally induced fatty liver in rats
Lipidomic, Eclipta prastvata, bouhinia retusa, fatty liver disease
Non-alcoholic fatty liver disease (NAFLD) is a leading cause of chronic liver disease world wide. Hundreds of plants have been examined for use in a wide variety of liver disorders; include Eclipta prostrata (E. prostrata) and Bauhinia Species which have several biological activities including hepatoprotective, hypolipidemic and antioxidant effects. The current study aimed to detect and evaluate lipidomic biomarkers in both serum and urine samples of rats for early detection of NAFLD and the potential efficiency of methanolic extract of E.prostrata and Bauhinia retusa (B. retusa) on disease management. Toxicity assays proved the safety effect of the methanolic extract of E.prostrata aerial part (stem and leaves) in contrast to B.retusa seeds methanolic extract which significantly increases the liver function tests and lipid profile in normal rats. So, B.retusa methanolic extract was excluded from this study. Phytochemical study of E. prostrata extract was performed using chemical colorimetric analysis and HPLC which was used also, to determine polyphenols and flavonoids compounds. Rats were divided into 3 main groups; Control groups (GI) included 24 rats divided into 2 subgroups: normal healthy control (GIa): 12 normal rats received standard chow (SC) and tap water ad libitum, half of them were sacrificed after 8 weeks as a control group of protected groups and the other half were sacrificed after 12 weeks as a control group of treated groups. Control with induced fatty liver (GIb): 12 normal rats fed a high-fat diet containing SC supplemented with cholesterol and cholic acid, half of them were sacrificed after 8 weeks as a control group of protected groups and the other half were sacrificed after 12 weeks as a control group of treated groups. Protected group (GII) included 30 rats and divided into 2 sub-groups: E.prostrata protected groups (GIIa): 24 normal rats fed high-fat diet containing SC supplemented with cholesterol and cholic acid followed by oral administration of methanolic extract of E.prostrata at doses (50, 100, 200, 300 mg/kg BW) for 8 weeks respectively, at the same time (6 rats for each dose). Lipanthyl drug protected group (GIIb): 6 normal rats received a high-fat diet containing SC supplemented with cholesterol and cholic acid followed by the lipid-lowering agent Lipanthyl® drug 300mg at a dose 5.35 mg/200g BW. Treated groups (GIII) included 30 rats divided into 2 sub-groups: E. prostrata treated groups (GIIIa): 24 normal rats fed a high-fat diet containing SC supplemented with cholesterol and cholic acid for 8 weeks, followed by oral administration of methanolic extract of E. prostrata (50, 100, 200, 300 mg/kg BW) respectively, for extra 4 weeks (6 rats for each dose). Lipanthyl treated group (GIIIb): 6 normal rats fed a high-fat diet containing SC supplemented with cholesterol and cholic acid for 8 weeks, followed by oral administration of the lipid-lowering agent Lipanthyl® drug 300 mg at a dose 5.35 mg/200g BW for extra 4 weeks. Urine samples were collected from all rats and stored at -80 C for lipidomic assay. Sera were aliquoted, stored at -80 C and used to determine liver, kidney function tests, lipid profile and lipidomic assay. Histopathological investigations of liver sections were studied in all groups.
2019
Ph.d
Ain Shams
Science